ISOLATION OF 2 ANTIGENS FROM THE CULTURE FILTRATES OF MYCOBACTERIUM-TUBERCULOSIS AND THEIR APPLICATIONS IN THE LABORATORY DIAGNOSIS OF THE TUBERCULOUS MENINGITIS
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Date
1991
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MEDICAL MICROBIOLOGY AND IMMUNOLOGY
Abstract
Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzyme-linked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than IAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.
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Immunology
Citation
MEDICAL MICROBIOLOGY AND IMMUNOLOGY. 180; 2; 101-107