Browsing by Author "Ramesh Babu, V"

Now showing 1 - 7 of 7
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    3D printing of liver constructs for in vitro hepatotoxicity testing ( Project - 8141 )
    (SCTIMST, 2021-08-15) Anil Kumar, PR; Shiny, Velayudhan; Roy, Joseph; Kalliyna Krishnan; Kumary, TV; Anugya, Bhatt; Lynda V, Thomas; Sarath S, Nair; Ramesh Babu, V; Sivaram, S; Praveen, KS
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    Examination Booth (Project - 8230)
    (SCTIMST, 2020-05-21) Ramesh Babu, V; Muraleedharan, CV; Arvind Kumar Prajapati; Saurabh S Nair; Sivakumar, KGV
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    Fabrication of co-cultured tissue constructs using a dual cell seeding compatible cell culture insert with a clip-on scaffold for potential regenerative medicine and toxicological screening application
    (Journal of Science: Advanced Materials and Devices, 2020-06) Ameer, JM; Ramesh Babu, V; Vinod, D; Nishad, KV; Sabareeswaran, A; Anil Kumar, PR; Kasoju, N
    Tissue engineering is emerging as a modern medicine fascination towards the establishment of human tissue banks; yet, these approaches typically involve cultures of only one type of cell and, therefore, do not recapitulate the native tissue physiology in toto. Co-culture models, comprised of different cell types, can potentially create the next level of complexity. However, conventional approaches involving multiple cell types and cell culture inserts do have limitations. To this end, here we demonstrate a novel cell culture insert that allows the use of any custom-made scaffold, free-flow of fluids/gases, dual cell seeding on either sides of the insert, easy stacking of multiple inserts and resizing it to any multi-well plate format as well as culture dishes. To prove the concept, electrospun silk fibroin scaffold was clipped onto the insert and was used for co-culturing of keratinocytes and fibroblast cells. The results indicated a successful fabrication of spatially organized skin tissue constructs having epidermal and dermal equivalent histology. Cell-laden inserts were stacked and used for simulated transportation studies. However, the conditions need further fine-tuning. All together, the results indicated that the novel cell culture insert with silk fibroin scaffold could be used as a facile, versatile and scalable approach to fabricate and transport 3D co-cultured tissue constructs in vitro, including but not limited to skin. The resultant tissue constructs can be explored for therapeutic applications, for instance as artificial skin substitute in wound healing, and for toxicological applications, for instance as reconstructed skin tissue model in skin irritation testing.
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    Graphene−Gold NanohybridBased Surface-Enhanced Raman Scattering Platform on a Portable Easyto-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells
    (ACS Sustainable Chemistry and Engineering, 2021-11) Jibin, K; Ramesh Babu, V; Jayasree, RS
    Although, cancer is considered as a localized disease in its premature stages, in certain types of cells, it results in metastasis, which accounts for over 90% of the total cancer deaths in the world. During the metastatic stage, cancer cells migrate from the primary tumor site to secondary sites through the circulating bloodstream, resulting in metastasis at unexpected body parts. This situation makes it critical to identify and quantify the circulating tumor cells (CTCs) during the early stages of tumorigenesis. Despite the clinical importance and progress of liquid biopsy and CTC-based cancer diagnostics, it still remains extremely challenging to develop systems to detect CTCs in cancer patients as its presence is extremely low in number (1–10 CTCs per mL of blood plasma). In this article, we discuss about the design and fabrication of a robust and user-friendly custom-designed nanotag-enabled portable filter-based sensor system for the selective separation and isolation of circulating breast cancer cells from whole blood. The filter sensor platform basically consists of a centrifugal prototype to quickly transfer unprocessed blood samples up to 5 mL within 60 s, isolating the circulating breast cancer cells selectively over the anti-EpCAM antibody-immobilized polycarbonate (PC) filter. Moreover, it is also equipped with a sandwich complex of a surface-enhanced Raman scattering (SERS) nanotag comprising a gold–graphene hybrid integrated with anti-ErbB2 antibody (Au-rGO@anti-ErbB2) for the SERS-assisted quantification of CTCs. As compared to the immunoaffinity-based CTC isolation platforms, the centrifugal force-based SERS nanotag enabled filter sensor system proved to be a clog free, highly sensitive, and selective platform toward the rapid isolation of viable breast cancer cells up to 5 tumor cells/mL from whole blood without any sample pretreatment. The high sensitivity, ease of use, and quick availability of results recommends the current filter sensor platform as a universal liquid biopsy technique for the quantification of CTCs.
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    Preparation and standardization of a batch of Reference Biomaterials (RM) for biological evaluations ( Project - 8169 )
    (SCTIMST, 2020-11-27) Leena, Joseph; Ramesh, P; Remya, NS; Ramesh Babu, V; Anil Kumar, PR; Anugya, Bhatt; Sabareeswaran, A
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    A versatile approach for temporary storage and shipping of in vitro cultured cells, cell sheets and tissue engineered constructs – a preliminary report
    (Engineered Regeneration, 2022-09) Anju, MS; Athira, RK; Ramesh Babu, V; Anil Kumar, PR; Kasoju, N
    Temporary storage/ shipping of cell/ tissue engineering products from bench to bedside is a key aspect of regenerative medicine. The current proof-of-concept study presents a multipurpose device for temporary storage/ shipping of cell culture dishes containing cell/ tissue constructs. The device, made with readily available raw materials, contains three elements viz. a specialized lid, polymeric plates having grooves and a set of nuts and bolts. As part of the performance evaluation, the device was first subjected to a simulated storage/ shipping process, wherein the leak-proof and aseptic containment of the contents was demonstrated. Subsequently, the setup was used for temporary storage/ shipping of dishes having (a) L929 cell monolayers cultured on treated surfaces, (b) SIRC, HaCaT and A549 cell sheets cultured on thermo-responsive surfaces, (c) HOS-cell encapsulated agar gels and (d) HOS-cell seeded silk fibroin mats. The results showed that the health of cell monolayers/ cell sheets/ tissue constructs after the process was comparable to that before the process. The device was scalable, simple to handle, can be made for a single or multi-use purpose, and can be resizable to load other culture vessels. The design of the storage/ shipping device described in this report thus offers versatile features and applications.