Browsing by Author "Sukanya, VS"

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    Complicity of degradable polymers in health-care applications
    (Materials Today Chemistry, 2020-01) Anju, S; Prajitha, N; Sukanya, VS; Mohanan, PV
    Polymeric biomaterials have revolutionized biomedical technology and related fields as biomaterials for health-care applications. Recent trend in polymeric medical technology has adapted a tendency to substitute degradable polymers instead of non-degradable synthetic polymers for the advancement of various health-care modalities. They have got considerable attention for their potential in various interdisciplinary arenas, which implies tissue engineering scaffolds, sustainable drug release, delivery agents, regenerative medicine, and development of life-saving devices, implants, dental products as well as in food technology. Various types of degradable polymers are been developed to date having stringent features applicable for various aspects in modern science. Thus, being the most renovative field of biomedicine and biomedical technology degradable polymers has gained substantial acceptance and appreciation recent times. This review critically underlines various degradative polymers and their subtypes, potential applications, types of degradation, and their possible effects in the biological system. Assessment of possible toxicological risks behind is an important criterion to be focused before validating any biomaterial safe for biomedical applications. Therefore various toxicological assessment strategies and their impact in biomedicine and technology were also included. In addition, the risk versus benefit assessment is also critically summarized.
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    Degradation of Poly(-caprolactone) and bio-interactions with mouse bone marrow mesenchymal stem cells
    (Colloids and Surfaces B: Biointerfaces., 2018-01) Sukanya, VS; Mohanan, PV
    Bio-inspired scaffolds in bone tissue engineering using multipotential mesenchymal stem cells grow at a rapidrate foundits successfuluse inorthopedic injury treatment. Poly(-caprolactone)/PCL is widelyused in medical devices, tissue engineering, and drug delivery systems. Most desirable property of biodegradable polymer to be employed in medical application is synchronization of degradation with functional tissue regeneration. Limited studies have incorporated the degradation kinetics and implication of degradation products of pure unmodified PCL. The present study analyzes shortterm in vitro degradation profile of PCL films in physiological condition. The study reports weight loss, changes in molecular weight distribution and morphological variation in PCL thin film over a period of 90-day degradation. When the degradable material is in contact with host tissue, there exists robust and dynamic microenvironment controlling the cell functionality. To comprehend the biocompatibility aspects of polymer material, the study considered mouse bone marrow mesenchymal stem cells (BMSCs) as model system mimicking in vivo. There was no indication of toxicity revealed with MTT, LDH leakage, direct contact assay and clonogenic assay. Absence of oxidative stress and apoptosis denotes BMSCs functional integrity sustained upon exposure to PCL degradation products. Cell cycle analysis and DNA ladder assay confirmed cell survival and genomic stability. The study revealed that the topography of pure unmodified PCL surface is suitable for cell adhesion. It was also observed that the viability of differentiated cells (osteoblasts) was maintained in presence of PCL extract. Furthermore, polymer and its degradation products were proved to be hemocompatible. These results synergistically suggestthat pure unmodified PCL and its degradation products are non-toxic at molecular level.