Calcium ion modulates protein release from chitosan-hyaluronic acid poly electrolyte gel
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Date
2015-05
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Poly. Eng. Sci.
Abstract
Polyelectrolyte complex (PEC) of chitosan (CH) and hyaluronic
acid (HA) are widely used for skin, cartilage, and
bone tissue engineering. However, no reports are seen on
their response at high ionic media, like increased Ca21
where they are likely to be exposed in the form of bone
constructs and the influence of these ions on modulating
the release of incorporated entities such as drugs and
growth factors. Here, we prepared freeze dried scaffolds
of PEC of CH and HA (CH-HA) and characterized them by
FTIR, TGA, SEM, and ESEM. FITC conjugated BSA, designated
as FA, was incorporated into the PEC to study the
release properties in response to Ca21. The swellability of
CH-HA and the extent of drug release from the matrix, FA
loaded CH-HA was studied in deionised water and aqueous
Na1 and Ca21 solutions. Swelling and drug release
were high for the matrix in aqueous Ca21 whereas it was
remarkably low in water and Na1. Drug released was
found to increase with concentrations of Ca21 (0.02–1.0M)
indicating that CH-HA is a promising matrix for Ca21
responsive delivery of agents to accelerate healing of
bone cracks, which is known to release high amount of
Ca21.
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Citation
Krishna AS , Radhakumary C, Sreenivasan K. Calcium ion modulates protein release from chitosan-hyaluronic acid poly electrolyte gel. Poly. Eng. Sci. 2015.