Induction of oxidative stress and lymphocyte proliferation by nanohydroxyapatite in mice
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Date
2015-06
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Basic & Applied Research International
Abstract
The aim of this study is to evaluate the potential impact of intraperitoneally administered nanohydroxyapatite on
the liver oxidative status and also to study the immunogenicity of the administered nanohydroxyapatite in Swiss
albino mice. Biochemical estimation of the lipid peroxidation, reduced glutathione and the activities of the
Glutathione peroxidase, Glutathione reductase and the Superoxide dismutase in the liver were carried out
following 7, 14 and 21 days post-treatment with nanohydroxyapatite. Tritiated thymidine uptake studies were
done with the splenic lymphocytes for studying the immunogenic potential. Histopathological evaluation of the
liver, spleen and the kidney was also carried out. There was marked reduction in the reduced glutathione levels
and in the activities of the antioxidant enzymes GPX, GR and SOD at 7 and 14 day and further showed an
increase by the 21 day. The LPO levels showed a significant increase by the 21 day. The T lymphocytes
proliferated by two-fold by the 7 day and returned to normal levels by the 14 day and an increase was seen by
the 21 days. There was no histopathological lesion observed in the treated groups. The nanohydroxyapatite
treatment lead to a marked reduction in the levels of the antioxidant enzyme activities which however increases
by the 21 day indicating that the body is able to defend against the oxidative stress mounted by the
nanohydroxyapatite. Nanohydroxyapatite has immunogenic potential as it was able to elicit proliferation of the T lymphocytes up to 21 days.
Description
Keywords
Nanohydroxyapatite (nano HA); oxidative stress; immunogenicity; nanoparticles; antioxidants; lipid peroxidation
Citation
Lekshmy M S, Gayathri V, Sabareeswaran A, Mohanan P V. Induction of oxidative stress and lymphocyte proliferation by nanohydroxyapatite in mice. Journal of Basic & Applied Research International. 2015;8(3):2395-3446.