Synthesis And Characterization of Pegylated Reduced Graphene Oxide: Determination of Toxicity Using Bone Marrow Mesenchymal Stem Cells
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Date
2015-10
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Applied Chemical Science International.
Abstract
Graphene is novel class of carbon based nanomaterial that has several unique physico-chemical characteristics.
These properties are being exploited in the biomedical field especially stem cell regenerative therapy. Due to
their superior mechanical strength, ability to induce stem cell differentiation and proliferation and antibacterial
properties, it is used as a coating for tissue engineered scaffolds. However there is a scarcity of literature on
biocompatibility of graphene. The aim of this study is to synthesize PEGylated reduced graphene oxide (PrGO)
and assess its biocompatibility in bone marrow mesenchymal stem cells (MSCs). PrGO was synthesized by
reduction of graphene oxide and characterized using TEM, SAED, AFM, XPS, Raman spectroscopy and FTIR.
MSCs were subjected to characterization to evaluate their stemness. The characterized MSCs were exposed to
varying concentration of PrGO and MTT assay was carried out. It was found that PEG was successfully coated
on to rGO and MSCs maintained their stemness in vitro. Moreover, the PrGO was found to be biocompatible
and increase proliferation of MSCs. In conclusion, in-house synthesized PrGO was found to be non toxic when
exposed to MSCs. This study can help design PrGO for safe biomedical applications.
Description
Keywords
PEGylated reduced graphene oxide; mesenchymal stem cell; nanomaterial characterization; stem cell characterization.
Citation
Syama S, Reshma S. Cherian RS, Poulose AC, Maekawa T, D. Sakthikumar D, Mohanan PV. Synthesis And Characterization of Pegylated Reduced Graphene Oxide: Determination of Toxicity Using Bone Marrow Mesenchymal Stem Cells. Journal of Applied Chemical Science International. 2015;5(1):1-11.