Modified polyacrylamide microspheres as immunosorbent
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Date
1997
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Publisher
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY
Abstract
Extracorporeal immunoadsorption system has been used for the specific removal of immunologically active substances from the blood. In this study, an attempt is made to utilise polyacrylamide microspheres as a matrix for immunoadsorption. Phenyl alanine is coupled onto the polymer beads using glutaraldehyde. These modified beads exhibit a high binding affinity for gamma-globulin compared to bare beads. The surface modified ones showed selective adsorption of immunoglobulins of IgG class. The C-3 adsorption pattern is not altered significantly upon modification. Modified beads are found to be less hemolytic after modification. However, more studies are to be conducted for the development of a hemoperfusion column based on this modified matrix.
Description
Keywords
Biomaterials
Citation
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY. 25; 6; 541-550