Molecular toxicity of dextran coated ferrite nanoparticles after dermal exposure to Wistar rats
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Date
2014-03
Journal Title
Journal ISSN
Volume Title
Publisher
J of Toxicology and Health
Abstract
Dextran coated ferrite nanomaterials (DFNM) of
size <25 nm was synthesized, characterized and
analyzed for systemic toxicity at the molecular level
by in vitro and in vivo methods. Skin, the external
barrier is one of the main portals of nanoparticles
entry upon accidental exposure in work place. In the
present study, DFNM was dermally applied on the
skin of Wistar rats to monitor the translocation of
nanomaterials from the route of entry and
subsequent systemic toxicity. At the end of 28 days
of exposure, blood was collected and subjected to
haematological and biochemical analysis. Gross
necropsy was conducted and major organs were
collected for histopathological observations. Liver
samples of both the in vitro and in vivo studies were
evaluated for the production of LPO, activity of GR,
GPx, SOD, total GSH and level of 8-OHdG formed.
The results demonstrated that there were no
significant fluctuations in the level of antioxidant
systems, lipid peroxidation, haematological,
biochemical, or histopathological lesions. Least
amount of increase in 8-OHdG was observed in the
in vitro samples. Hence, it can be concluded that
the synthesized DFNM was non-toxic on dermal
exposure and fails to induce any potential damages
in the vital organs functions.
Description
Keywords
Dextran, Nanomaterials, DNA damage, Dermal Toxicity, Oxidative Stress
Citation
J of Toxicology and Health. 2014;104:406-22